Render Target: SSR
Render Timestamp: 2024-10-24T19:59:00.801Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-04-24 09:10:53.374
Product last modified at: 2024-10-07T21:00:09.711Z
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PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Goat Anti-Rat IgG, Light-Chain Specific Antibody (HRP Conjugate) #98164

Filter:
  • WB

    Supporting Data

    REACTIVITY R
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Description

    Affinity purified Goat Anti-Rat IgG, Light-Chain Specific antibody is conjugated to horseradish peroxidase (HRP). This product has been optimized for use as a secondary antibody in Western blotting applications.

    Product Usage Information

    Recommended Antibody Dilutions:

    1:3000 - 1:5000

    Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.

    Storage

    Supplied in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease- Free) and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Goat Anti-Rat IgG, Light-Chain Specific Antibody (HRP Conjugate) detects the light chains of rat IgG, reacting primarily with kappa light chains, but does not cross-react with rat IgG heavy chains. This antibody has minimal cross-reaction with bovine, goat, horse, human, mouse, rabbit, and sheep immunoglobulins. It may cross-react with immunoglobulins from other species.

    Species Reactivity:

    Rat

    Source / Purification

    Goat Anti-Rat IgG, Light-Chain Specific Antibody (HRP Conjugate) is produced by immunizing goats with rat IgG. The Anti-Rat IgG, Light-Chain Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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