Goat Anti-Mouse IgG3 Antibody (AP Conjugate) #96356
Filter:
- WB
Western blot analysis of mouse IgG subclasses (10 ng per lane) reduced and denatured in 1x SDS loading buffer with DTT using Goat Anti-Mouse IgG3 Antibody (AP Conjugate) (left) or Anti-mouse IgG, AP-linked Antibody #7056 (right).
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
- Related Products
Product Information
Product Description
Affinity purified Goat Anti-Mouse IgG3 antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.
Product Usage Information
Recommended Antibody Dilutions:
1:2000 - 1:8000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
1:2000 - 1:8000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Stable for 12 months when stored at -20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Goat Anti-Mouse IgG3 Antibody (AP Conjugate) detects the heavy chain of mouse IgG3. This antibody has been cross-adsorbed against mouse IgG1, IgG2a, IgG2b, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.
Species Reactivity:
Mouse
Source / Purification
Goat Anti-Mouse IgG3 Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG3. The Anti-Mouse IgG3 antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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