Goat Anti-Mouse IgG1 Antibody (AP Conjugate) #48253
Filter:
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Affinity purified Goat Anti-Mouse IgG1 antibody is conjugated to alkaline phosphatase (AP). This product has been optimized for use as a secondary antibody in Western blotting applications.
Product Usage Information
Recommended Antibody Dilutions:
1:2000 - 1:8000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
1:2000 - 1:8000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Supplied in 50 mM Tris, 1 mM MgCl2, pH 8.0, and 50% glycerol containing < 0.1% sodium azide. Store at -20°C. Do not aliquot the antibody.
Specificity / Sensitivity
Goat Anti-Mouse IgG1 Antibody (AP Conjugate) detects the heavy chain of mouse IgG1. This antibody has been cross-adsorbed against mouse IgG2a, IgG2b, IgG3, IgM, and IgA, human, bovine, and horse serum proteins. It may cross-react with immunoglobulins from other species.
Species Reactivity:
Mouse
Source / Purification
Goat Anti-Mouse IgG1 Antibody (AP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1 antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages of AP conjugation is that the reaction rate remains linear for a long period of time.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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