Anti-rat IgG (H+L), (Alexa Fluor® 555 Conjugate) #4417
Filter:
- IF
Supporting Data
REACTIVITY | R |
SENSITIVITY | |
MW (kDa) | |
Source/Isotype | Goat |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- R-Rat
Product Information
Product Description
Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/mL.
Product Usage Information
The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 - 1:2000 should yield acceptable results for immunofluorescent assays.
Storage
Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum. Cross-reactivity with mouse primary antibodies has been observed.
Species Reactivity:
Rat
Background
This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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