渲染靶标:SSR
Render Timestamp:
4/2/2025, 12:32:18 PM EDT
4/2/2025, 4:32:18 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:09:42.523
Product last modified at: 2025-03-20T12:00:38.783Z
1% for the Planet 标识
PDP - Template Name: Secondary Antibody
PDP - Template ID: *******76815a7

Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4413

Filter:
  • IF
  • F
Immunofluorescence Image 1: Anti-rabbit IgG (H+L), F(ab')<sub>2</sub> Fragment (Alexa Fluor® 555 Conjugate)
Confocal immunofluorescent analysis of mouse olfactory bulb using Calbindin (D1I4Q) XP® Rabbit mAb #13176 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4413 (red) and Neurofilament-L (DA2) Mouse mAb #2835 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4410 (yellow pseudocolor). Sections were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

To Purchase # 4413

Supporting Data

REACTIVITY Rab
SENSITIVITY
MW (kDa)
Source/Isotype Goat
Application Key:
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • Rab-Rabbit 
  • Related Products

Product Information

Product Description

Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500–1:2000 should yield acceptable results for immunofluorescent and flow cytometry assays.

Storage

Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Specificity / Sensitivity

F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Species Reactivity:

Rabbit

Background

This product has been optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for Immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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