Anti-mouse IgG (H+L) was conjugated to DyLight 800 4X PEG fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 777 nm and peak fluorescence emission is 794 nm.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.

Fluorescent western blotting: 1:15000

In-Cell Western: 1:2000

Storage

Proprietary buffer. Store at 4°C. Protect from Light. Do not freeze.

Protocol

Specificity / Sensitivity

Anti-mouse IgG (H+L) (DyLight 800 4X PEG Conjugate) reacts with the heavy and light chains of most mouse immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.

Species Reactivity:

Mouse

Source / Purification

This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.

Background

Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.

限制使用

除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受，否书均被拒书，并且无效。

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Anti-mouse IgG (H+L) (DyLight 800 4X PEG Conjugate) #5257

Supporting Data

Application Key:

Species Cross-Reactivity Key:

Product Information

Product Description