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Anti-mouse IgG (H+L) (DyLight 680 Conjugate)

Anti-mouse IgG (H+L) (DyLight 680 Conjugate) #5470

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Supporting Data

REACTIVITY	M
SENSITIVITY
MW (kDa)
Source/Isotype	Goat

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

M-Mouse

Product Information

Product Description

Anti-mouse IgG (H+L) was conjugated to DyLight 680 fluorescent dye under optimal conditions and formulated at 1 mg/ml. Excitation is 684 nm and peak fluorescence emission is 715 nm.

Product Usage Information

The optimal dilution of the anti-species antibody should be determined by the user. However, the final dilutions below should yield acceptable results for the respective applications.

Fluorescent western blotting: 1:15000

In-Cell Western: 1:1000

Storage

Supplied in 100 mM PBS, pH 7.2, containing 1% BSA and 0.02% sodium azide. Store at 4°C. Protect from Light. Do not freeze.

Protocol

Specificity / Sensitivity

Anti-mouse IgG (H+L) (DyLight 680 Conjugate) reacts with heavy and light chain of most mouse immunoglobulins. No cross-reactivity to other serum proteins has been detected. This antibody may cross-react with immunoglobulins from other species.

Species Reactivity:

Mouse

Source / Purification

This antibody is prepared from goat antibodies and purified by immunoaffinity chromatography using antigen coupled to agarose beads.

Background

Near infrared anti-species IgG conjugates are ideal for fluorescent western blotting and In-Cell Western. Cell Signaling Technology's strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
This product has been optimized for use as a secondary antibody in fluorescent western blotting and In-Cell Western™.

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

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