R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ZNF217 (E6X4R) Rabbit mAb #82306
Filter:
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ZNF217 (E6X4R) Rabbit mAb recognizes endogenous levels of total ZNF217 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZNF217 protein.
Background
Zinc finger protein 217 (ZNF217) is an oncogenic transcription factor that regulates the expression of a wide variety of genes. ZNF217 is a member of the Kruppel-like factor (KLF) family of transcription factors, which consists of zinc finger proteins that bind to CACCC or GT box DNA elements (1). ZNF217 regulates the expression of its target genes through direct DNA binding, and through interaction with transcriptional co-repressors in the CoREST-HDAC complex (2). Although it was initially described as a transcriptional repressor, ZNF217 may also act as a transcriptional activator depending on the target gene (3). Amplification of the ZNF217 gene itself has been observed in a variety of tumors, where it is typically associated with poor prognosis (4-7). Overexpression of ZNF217 protein leads to increased cell proliferation and promotes tumor growth in breast and ovarian cancer (8,9). ZNF217 also interferes with apoptotic signaling and confers resistance to chemotherapeutic drugs in breast cancer (9,10).
- Pollak, N.M. et al. (2018) JACC Basic Transl Sci 3, 132-156.
- Cowger, J.J. et al. (2007) Oncogene 26, 3378-86.
- Cohen, P.A. et al. (2015) Oncotarget 6, 41566-81.
- Rooney, P.H. et al. (2004) J Pathol 204, 282-8.
- Rahman, M.T. et al. (2012) Cancer 118, 2846-57.
- Rahman, M.T. et al. (2012) Anticancer Res 32, 3091-5.
- Mao, X.G. et al. (2011) Lab Invest 91, 1068-78.
- Li, J. et al. (2014) Int J Clin Exp Pathol 7, 3038-47.
- Thollet, A. et al. (2010) Mol Cancer 9, 291.
- Huang, G. et al. (2005) Hum Mol Genet 14, 3219-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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