R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ZIP7/SLC39A7 (D1O3A) Rabbit mAb #33176
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ZIP7/SLC39A7 (D1O3A) Rabbit mAb recognizes endogenous levels of total ZIP7 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu201 of human ZIP7 protein.
Background
The solute carrier family 39 (zinc transporter) member 7 (SLC39A7, ZIP7) is an ER and Golgi membrane protein that regulates cellular zinc homeostasis by controlling release of zinc from these organelles to the cytoplasm (1,2). Zinc release mediated by ZIP7 results in activation of protein kinases that are involved in cell proliferation and migration (3,4). The protein kinase CK2 phosphorylates and activates ZIP7 in response to extracellular signals, such as growth factor stimulation (4,5). Increased expression of ZIP7 is observed in breast cancer tissues (6). Research studies indicate that ZIP7 is responsible for activation of multiple tyrosine kinases in aggressive, tamoxifen-resistant breast cancer (7,8).
- Taylor, K.M. et al. (2004) Biochem J 377, 131-9.
- Huang, L. et al. (2005) J Biol Chem 280, 15456-63.
- Hogstrand, C. et al. (2009) Trends Mol Med 15, 101-11.
- Taylor, K.M. et al. (2012) Cell Cycle 11, 1863-4.
- Taylor, K.M. et al. (2012) Sci Signal 5, ra11.
- Taylor, K.M. (2008) Biochem Soc Trans 36, 1247-51.
- Taylor, K.M. et al. (2007) Mol Med 13, 396-406.
- Taylor, K.M. et al. (2008) Endocrinology 149, 4912-20.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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