Render Target: SSR
Render Timestamp: 2025-04-01T19:22:43.289Z
Commit: 779953b12a5930618aae6aca7c87fb286faeb1d7
XML generation date: 2025-03-07 13:18:51.390
Product last modified at: 2025-03-17T19:00:09.487Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ZIP14/SLC39A14 (E3H7D) Rabbit mAb #24161

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: ZIP14/SLC39A14 (E3H7D) Rabbit mAb
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ZIP14/SLC39A14 (hZIP14/SLC39A14; +), using ZIP14/SLC39A14 (E3H7D) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 24161

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 55-65
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:800 - 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ZIP14/SLC39A14 (E3H7D) Rabbit mAb recognizes endogenous levels of total ZIP14/SLC39A14 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu282 of human ZIP14/SLC39A14 protein.

Background

Solute carrier (SLC) family member Zrt- and Irt-like protein 14 (ZIP14) is a transmembrane metal ion symporter encoded by the gene SLC39A14. ZIP14 functions as a plasma membrane transporter of zinc and manganese, and as an endosomal promoter of intracellular uptake of iron from transferrin (1). In liver, ZIP14 localizes to the basolateral membrane of hepatocytes, transporting manganese from the blood for eventual delivery to the bile duct (2). In pancreatic beta cells, SLC39A14 expression is glucose-sensitive and plays a key role in zinc-dependent insulin secretion (3).

The reported ZIP14 L441R mutation (L438R in mice) disrupts plasma membrane presentation of the transporter, causing intracellular accumulation of zinc in osteoblasts, characterized by excessive bone overgrowth (4).

SLC39A14 mutation or knockdown can cause manganese dyshomeostasis, resulting in childhood-onset dystonia-Parkinsonism (5). The activity of liver-expressed ZIP14/SLC39A14, intestinal SLC39A10, and blood-brain barrier ZIP8/SLC39A8 all contribute to manganese homeostasis during neuronal development (6,7).
For Research Use Only. Not For Use In Diagnostic Procedures.
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