ZDHHC5 (E7T4N) Rabbit mAb #79842

Zinc finger DHHC domain-containing protein 5 (ZDHHC5) is a multipass membrane protein that is localized predominantly at the plasma membrane and endosomal compartment membranes (1). ZDHHC5 belongs to a large family of enzymes known as palmitoyl-acyltransferases. Using long-chain fatty acids such as palmitic acid, ZDHHC5 catalyzes the reversible, post-translational modification of cysteine residues within protein substrates to regulate their trafficking and subcellular localization (1,2). Research studies have shown that ZDHHC5 is itself regulated by multiple types of post-translational modifications, such as phosphorylation and palmitoylation, which regulate its subcellular localization and enzymatic activity (3,4). Palmitoylation mediated by ZDHHC5 plays a role in the host response to pathogens. Indeed, ZDHHC5-mediated palmitoylation of NOD1/2 pattern recognition receptors facilitates their correct localization to endosomal vesicle membranes containing internalized bacteria in order to initiate innate immune signaling cascades (5). Furthermore, ZDHHC5 was recently shown to interact with SARS-CoV-2 spike protein. This interaction is posited to facilitate S-acylation of the spike protein and fusion with host cell membranes (6,7).