R Recombinant
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ZBTB24 (E5D6G) Rabbit mAb #15969
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ZBTB24 (E5D6G) Rabbit mAb recognizes endogenous levels of total ZBTB24 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro569 of human ZBTB24 protein.
Background
Zinc finger and BTB domain-containing protein 24 (ZBTB24), also called ZNF450, is a DNA-binding protein initially found to be expressed in response to BMP-2 signaling (1). ZBTB24 along with CDCA7 play a role in DNA methylation by recruiting the HELLS chromatin remodeling complex to centromeric satellite repeats (2). Mutations in ZBTB24 are linked to immunodeficiency and centromeric instability, and facial anomalies syndrome type 2 (ICF2) (3,4). ZBTB24 is recruited to DNA breaks by PARP1 and promotes assembly of the LIG4/XRCC4 complex, resulting in error free non-homologous end joining (NHEJ) (5). ZBTB24 has also been shown to play a role in trophoblast proliferation through the methylation of E-cadherin (6)
- Edgar, A.J. et al. (2005) J Cell Biochem 94, 202-15.
- Hardikar, S. et al. (2020) Protein Cell 11, 214-218.
- de Greef, J.C. et al. (2011) Am J Hum Genet 88, 796-804.
- Chouery, E. et al. (2012) Clin Genet 82, 489-93.
- Helfricht, A. et al. (2020) J Exp Med 217, e20191688. doi: 10.1084/jem.20191688.
- Ruan, H. et al. (2022) Bioengineered 13, 2777-2790.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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