XRN1 Antibody #70205
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
XRN1 Antibody recognizes endogenous levels of total XRN1 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1696 of human XRN1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
5’-3’ exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5’-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover (1,2). This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins (3). XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3’ mRNA fragments generated by RISC cleavage. This process does not require uncapping or deadenylation (4). XRN1 plays a significant role in viral RNA degradation (5). As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affect innate immunity and viral replication (6).
- Brown, J.T. et al. (2000) Genetics 155, 31-42.
- Long, R.M. and McNally, M.T. (2003) Mol Cell 11, 1126-8.
- Cougot, N. et al. (2004) J Cell Biol 165, 31-40.
- Orban, T.I. and Izaurralde, E. (2005) RNA 11, 459-69.
- Cheng, C.P. et al. (2006) J Virol 80, 2631-40.
- Charley, P.A. and Wilusz, J. (2016) Virus Res 212, 70-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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