R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
XRCC3 (E9X4D) Rabbit mAb #20026
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
XRCC3 (E9X4D) Rabbit mAb recognizes endogenous levels of total XRCC3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human XRCC3 protein.
Background
XRCC3 (X-ray repair cross-complementing protein 3) is one of five RAD51 paralogs that form two distinct complexes, BCDX2 (RAD51B, RAD51C, RAD51D, and XRCC2) and CX3 (RAD51C and XRCC3) (1). Along with RAD51, these complexes are crucial in the repair of DNA double-strand breaks through homologous recombination (2), and in the response to DNA replication stress (3,4). The CX3 complex also plays a role in maintaining mitochondrial DNA integrity during replicative stress (5). XRCC3 genetic polymorphisms are associated with risk and prognosis in human cancer (6-9).
- Masson, J.Y. et al. (2001) Genes Dev 15, 3296-307.
- Thacker, J. (2005) Cancer Lett 219, 125-35.
- Rein, H.L. et al. (2021) Curr Opin Genet Dev 71, 86-91.
- Berti, M. et al. (2020) Nat Commun 11, 3531.
- Mishra, A. et al. (2018) Mol Cell Biol 38, e00489-17. doi: 10.1128/MCB.00489-17.
- Zhao, Y. et al. (2019) J Oncol 2019, 2408946.
- Cheng, S. et al. (2015) Int J Clin Exp Pathol 8, 1911-9.
- Bei, L. et al. (2015) Bull Cancer 102, 332-9.
- Ozoran, E. et al. (2022) Int J Breast Cancer 2022, 5817841.
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