R Recombinant
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XRCC2 (E7M8Y) Rabbit mAb #86714
Filter:
- WB
- IP
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or XRCC2 siRNA (+), using XRCC2 (E7M8Y) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 29 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
XRCC2 (E7M8Y) Rabbit mAb recognizes endogenous levels of total XRCC2 protein. By western blot, this antibody detects a 40 kDa band of unknown origin.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys267 of human XRCC2 protein.
Background
XRCC2 (X-ray repair cross-complementing protein 2) is one of five RAD51 paralogs that form two distinct complexes, BCDX2 (RAD51B, RAD51C, RAD51D, and XRCC2) and CX3 (RAD51C and XRCC3) (1). Along with RAD51, these complexes are crucial in the repair of DNA double-strand breaks through homologous recombination (2) and in response to DNA replication stress (3,4). XRCC2 expression and genetic polymorphisms are associated with the risk and prognosis of human cancer (5-10). In non-small cell lung cancer (NSCLC) XRCC2 reduces sensitivity to chemotherapy and radiation treatment (11).
- Masson, J.Y. et al. (2001) Genes Dev 15, 3296-307.
- Thacker, J. (2005) Cancer Lett 219, 125-35.
- Rein, H.L. et al. (2021) Curr Opin Genet Dev 71, 86-91.
- Berti, M. et al. (2020) Nat Commun 11, 3531.
- Liu, Y. et al. (2021) J Cancer 12, 6254-6264.
- Liu, Z. et al. (2021) Mol Med 27, 52.
- Mandegari, M. et al. (2021) Asian Pac J Cancer Prev 22, 2221-2236.
- Wang, Z. et al. (2021) J Int Med Res 49, 3000605211039473.
- Gowtham Kumar, G. et al. (2021) Mol Biol Rep 48, 4209-4220.
- Yuan, C. et al. (2020) Arch Med Sci 16, 682-691.
- Shan, J. et al. (2022) Am J Transl Res 14, 3783-3795.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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