Render Target: SSR
Render Timestamp: 2025-03-06T19:36:04.184Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-12-06 15:01:07.098
Product last modified at: 2025-01-22T14:15:20.200Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

XLF Antibody #2854

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 39
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    XLF Antibody detects endogenous levels of total XLF protein.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residues surrounding Lys288 of human XLF protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Double stranded DNA breaks (DSB’s) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. The mechanism by which cells deal with DSB’s is known as NHEJ (non-homologous end-joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). XLF, also known as Cernunnos, was originally discovered as a mutated protein from cells of individuals who displayed features of growth retardation, microcephaly, and immunodeficiency (2). These cells were sensitive to ionizing radiation and defective in V(D)J recombination. Exogenous expression of wild type XLF corrected these deficiencies (3), indicating that XLF is a critical component of the NHEJ response. XLF physically interacts with and may stimulate the ligase activity of XRCC4 (3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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