xCT/SLC7A11 Antibody #98051
Filter:
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
xCT/SLC7A11 Antibody recognizes endogenous levels of total mouse xCT/SLC7A11 protein.
Species Reactivity:
Mouse
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of mouse xCT/SLC7A11 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The x(c)(-) cysteine/glutamate antiporter consists of a light chain subunit (xCT/SLC7A11) that confers substrate specificity and a glycosylated heavy chain subunit (4F2hc/SLC3A2) located on the cell surface (1,2). The heterodimeric amino acid transport system x(c)(-) provides selective import of cysteine into cells in exchange for glutamate and regulating intracellular glutathione (GSH) levels, which is essential for cellular protection from oxidative stress (3). Research studies have shown that xCT expression increases in various tumors, including gliomas, and have implicated xCT in GSH-mediated anticancer drug resistance (4,5). Researchers have found that xCT provides neuroprotection by enhancing glutathione export from non-neuronal cells (6). Moreover, investigators identified xCT as the fusion-entry receptor for Kaposi's sarcoma-associated herpesvirus (7).
- Sato, H. et al. (2000) Antioxid Redox Signal 2, 665-71.
- Sato, H. et al. (1999) J Biol Chem 274, 11455-8.
- Lo, M. et al. (2008) J Cell Physiol 215, 593-602.
- Huang, Y. et al. (2005) Cancer Res 65, 7446-54.
- Liu, R. et al. (2007) Mol Pharmacol 72, 1637-46.
- Shih, A.Y. et al. (2006) J Neurosci 26, 10514-23.
- Kaleeba, J.A. and Berger, E.A. (2006) Science 311, 1921-4.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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