R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
WWP1 (E1W4V) Rabbit mAb #70140
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 120 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
WWP1 (E1W4V) Rabbit mAb recognizes endogenous levels of total WWP1 protein. The antibody detects non-specific bands of unknown origin above 200 kDa.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human WWP1 protein.
Background
WWP1 (WW domain-containing protein 1, AIP5, Tiul1) is a NEDD4 family ubiquitin E3 ligase (1). The protein regulates many cellular processes, including protein trafficking and degradation, transcription, and signaling. WWP1 ubiquitination activity contributes to the progression of many diseases through polyubiquitination of crucial factors, such as cardiac hypertrophy via the WWP1/DVL2 pathway, Ebola virus egress via the WWP1/eVP40 pathway, and muscle atrophy via the WWP1/KLF15 pathway (2-4). Aberrant high expression of WWP1 is found in many types of tumors, including colorectal cancer, oral cancer, gastric cancer, hepatocellular cancer, and breast cancer (5). The upregulation of WWP1 activity enhances polyubiquitination of tumor suppressors, such as ΔNp63α, PTEN, p53, KLF5, and p27, and ultimately promotes cell proliferation, survival, migration, and invasion in tumorigenesis (6-10). Given the dysregulation and oncogenic functions of WWP1 in these diseases, the protein is considered as a promising therapeutic target.
- Zhi, X. and Chen, C. (2012) Cell Mol Life Sci 69, 1425-34.
- Zhao, D. et al. (2021) Circulation 144, 694-711.
- Han, Z. et al. (2017) J Virol 91, e00812-17. doi: 10.1128/JVI.00812-17.
- Hirata, Y. et al. (2019) JCI Insight 4, e124952. doi: 10.1172/jci.insight.124952.
- Hu, X. et al. (2021) Cell Death Discov 7, 163.
- Peschiaroli, A. et al. (2010) Biochem Biophys Res Commun 402, 425-30.
- Chen, C. et al. (2005) J Biol Chem 280, 41553-61.
- Lee, Y.R. et al. (2019) Science 364, eaau0159. doi: 10.1126/science.aau0159.
- Sanarico, A.G. et al. (2018) Leukemia 32, 911-919.
- Laine, A. and Ronai, Z. (2007) Oncogene 26, 1477-83.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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