VRK1 (1F6) Mouse mAb #3307
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
VRK1 (1F6) Mouse mAb detects endogenous levels of total VRK1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant fusion protein GST-full length human VRK1.
Background
The vaccinia-related kinase (VRK) proteins are a new group of Ser/Thr kinases in the human kinome. This mammalian kinase family comprises three members, VRK1, VRK2, and VRK3 (1-3). The VRK1 has autophosphorylation activity and phosphorylates several transcription factors, including p53 (4), ATF2 (5), and c-Jun (6). VRK2 is associated with the endoplasmic reticulum (7). VRK3 suppresses Erk activity through direct interaction and activation of the MAP kinase phosphatase VHR (8). Further functional and structural analysis of VRK proteins will elucidate important new aspects of cell regulation.
- Nezu, J. et al. (1997) Genomics 45, 327-31.
- Zelko, I. et al. (1998) Arch Biochem Biophys 352, 31-6.
- Vega, F.M. et al. (2003) FEBS Lett 544, 176-80.
- Lopez-Borges, S. and Lazo, P.A. (2000) Oncogene 19, 3656-64.
- Sevilla, A. et al. (2004) J Biol Chem 279, 27458-65.
- Sevilla, A. et al. (2004) Oncogene 23, 8950-8.
- Nichols, R.J. and Traktman, P. (2004) J Biol Chem 279, 7934-46.
- Kang, T.H. and Kim, K.T. (2006) Nat Cell Biol 8, 863-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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