VPS35 (E6S4I) Rabbit mAb #81453

Retromer is a hetero-pentameric protein complex that mediates retrograde transport of transmembrane proteins from endosomes to the trans-Golgi network (TGN). The retromer complex consists of two protein compounds, a sorting nexin dimer and a trimeric cargo-selective complex (CSC) composed of vacuolar protein sorting-associated protein 35 (VPS35), VPS26, and VPS29 (1-3). VPS35 is the largest of the three CSC proteins, and interacts with VPS26 through its amino terminus and with VPS29 through its carboxy terminus (1).

VPS35 and the retromer are involved in trafficking of specific cargo along the dendritic processes of neurons, including appropriate NMDA and AMPA receptor expression at synapses, and β2-adrenergic receptors (β2AR) outside of synapses and throughout the dendrite (4). It has been shown that VPS35 expression is required for survival of dopaminergic neurons through its role in mitochondrial fusion and function (5). Mutations in the VPS35 gene have been shown to cause late-onset, autosomal dominant familial Parkinson’s disease (6). VPS35 has also been implicated in Alzheimer’s disease by playing a role in regulation of Aβ peptide levels, with reduced protein levels of VPS35 seen in patient tissues (7).