R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
VIMP (D1D1M) Rabbit mAb #15160
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
VIMP (D1D1M) Rabbit mAb recognizes endogenous levels of total VIMP protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys150 of human VIMP protein.
Background
VCP-interacting membrane protein (VIMP, selenoprotein S) is a putative reductase and endoplasmic reticulum (ER)-resident protein involved in the ER-associated degradation (ERAD) pathway (1,2). Research studies indicate that VIMP may play a protective role against inflammation and reduce ER-stress (3). The VIMP protein is a single-pass, transmembrane protein that recruits the cytosolic p97/VCP AAA-ATPase and its cofactors, UFD1 and NPL4, to the ER membrane (4). An ER membrane complex containing Derlin-1 and VIMP forms a critical node in the ERAD machinery and links substrate recognition in the ER lumen with the retrotranslocation function of the p97/VCP AAA-ATPase in the cytosol (1,4). Polymorphisms in the corresponding VIMP gene are associated with spontaneous preterm births and cardiovascular disease risk (5,6) while other studies do not support a correspondence between VIMP polymorphisms and inflammatory disorders (7).
- Lilley, B.N. and Ploegh, H.L. (2005) Proc Natl Acad Sci U S A 102, 14296-301.
- Christensen, L.C. et al. (2012) J Biol Chem 287, 26388-99.
- Fradejas, N. et al. (2011) Glia 59, 959-72.
- Ye, Y. et al. (2004) Nature 429, 841-7.
- Wang, Y. et al. (2013) PLoS One 8, e65657.
- Cox, A.J. et al. (2013) Acta Diabetol 50, 391-9.
- Martínez, A. et al. (2008) BMC Genomics 9, 329.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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