R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Versican (F4U1K) Rabbit mAb #98586
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 700, 500, 400 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Versican (F4U1K) Rabbit mAb recognizes endogenous levels of total versican protein. This antibody detects all isoforms of versican protein. A smeary band of versican protein aggregates is observed at a very high molecular weight.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human versican protein.
Background
Versican (VCAN) is a large chondroitin sulfate proteoglycan (CSPG) located in the extracellular matrix (ECM). The core protein of versican has an N-terminal G1 domain for hyaluronan binding, followed by chondroitin sulfate binding domains, and a C-terminal G3 domain containing EGF-like repeats and a lectin binding region (1). These versican domains contribute to its interaction with other ECM molecules, including CD44 and integrin beta 1 (2). Versican is an important extracellular component for the formation of provisional matrix to mediate cardiac development, neural crest cell migration, and limb development (3,4). During inflammatory response, versican accumulates at the injury site and supports leukocyte adhesion and inflammatory cytokine production (5). During cancer development, overexpressed versican promotes cancer cell proliferation, survival, angiogenesis, invasion, and metastasis (6).
- Islam, S. and Watanabe, H. (2020) J Histochem Cytochem 68, 763-775.
- Wu, Y.J. et al. (2005) Cell Res 15, 483-94.
- Wight, T.N. (2017) Matrix Biol 60-61, 38-56.
- Nandadasa, S. et al. (2014) Matrix Biol 35, 34-41.
- Wight, T.N. et al. (2014) Matrix Biol 35, 152-61.
- Papadas, A. and Asimakopoulos, F. (2020) Adv Exp Med Biol 1272, 55-72.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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