R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
USP39 (E8U2M) Rabbit mAb #23303
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
USP39 (E8U2M) Rabbit mAb recognizes endogenous levels of total USP39 protein. This antibody may detect an 80 kDa band of unknown origin by western blotting. Cytoplasmic staining of uncertain specificity was observed in human brain by immunohistochemistry.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala367 of human USP39 protein.
Background
Ubiquitin specific protease 39 (USP39) is a 65 kDa protein that plays an important role in pre-mRNA splicing, as well as mitotic spindle formation. It displays significant homology with ubiquitin C-terminal hydrolase proteins (UCHs), containing both an N-terminal zinc finger domain as well as UCH-1 and UCH-2-like domains also observed in the UCH2 family of proteins (1). However, USP39 lacks a catalytic cysteine residue found in UCHs and has been shown experimentally to lack peptidase activity (2). USP39 associates with the U4/U6-U5 tri-small nuclear ribonucleoprotein (U4/U6-U5 tri-snRNP) complex and is necessary for the formation of the mature spliceosome. Silencing of USP39 has been shown to adversely affect chromosome segregation and cytokinesis in U2OS cells, likely due to improper splicing of Aurora B and other mRNAs necessary for mitotic spindle formation and checkpoint function (2). In addition, USP39 has been found to be overexpressed in many types of cancers, and in most cases is associated with tumor progression and poor prognosis. Overexpression has been observed in pancreatic (3), prostate (4), colorectal (5,6), lung (6,7), gastric (8), and triple negative breast cancers (9), as well as melanoma (10) and hepatocellular carcinoma (11,12).
- Makarova, O.V. et al. (2001) EMBO J 20, 2553-63.
- van Leuken, R.J. et al. (2008) Cell Cycle 7, 2710-9.
- Cai, J. et al. (2017) Biochem Biophys Res Commun 486, 184-90.
- Huang, Y. et al. (2016) Oncotarget 7, 22016-30.
- Yuan, X. et al. (2017) Oncol Rep 37, 2398-2404.
- Fraile, J.M. et al. (2017) J Biol Chem 292, 4164-75.
- Lin, Z. et al. (2016) Mol Cell Biochem 422, 97-107.
- Wang, X. et al. (2016) Mol Med Rep 14, 301-6.
- Liu, S. et al. (2015) Oncol Rep 33, 2477-83.
- Zhao, Y. et al. (2016) Tumour Biol 37, 13167-76.
- Yuan, X. et al. (2015) Oncol Rep 34, 823-32.
- Pan, Z. et al. (2015) Biol Res 48, 18.
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