UPP1 Antibody #48789
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 33 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UPP1 Antibody recognizes endogenous levels of total UPP1 protein. This antibody may also detect a non-specific band at approximately 45 kDa in a limited number of cell lines.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys174 of human UPP1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Uridine phosphorylases (UPP1, UPP2) are metabolic enzymes that catalyze the reversible phosphorolytic cleavage of uridine into uracil and ribose-1-phosphate, which can subsequently serve as cellular energy sources or as substrates for de novo nucleotide biosynthesis via pyrimidine salvage pathways (1). Altered pyrimidine metabolism has been reported in multiple cancer subtypes (2-5) and is hypothesized to be associated with cellular metabolic reprogramming events that enable enhanced tumor growth and metastasis (6,7). Notably, UPP1 was identified as one of the enzymes involved in the metabolic activation of the anti-neoplastic agent 5-fluorouracil (5-FU) (8,9). Somewhat paradoxically, numerous UPP1 inhibitors have been developed to reduce the adverse side effects of 5-FU treatment by elevating physiological levels of uridine during 5-FU administration (10).
- Pizzorno, G. et al. (2002) Biochim Biophys Acta 1587, 133-44.
- Sun, C. et al. (2019) Proc Natl Acad Sci USA 116, 52-57.
- Wang, J. et al. (2020) Cancer Med 9, 5940-5947.
- Yan, R. et al. (2006) Front Biosci 11, 2759-66.
- Liu, M. et al. (1998) Cancer Res 58, 5418-24.
- Lyssiotis, C.A. and Nagrath, D. (2019) Cancers (Basel) 12, 90.
- Wu, Z. et al. (2021) Cancer Cell Int 21, 668.
- Higley, B. et al. (1982) Gut 23, 1072-6.
- Renck, D. et al. (2010) Arch Biochem Biophys 497, 35-42.
- Darnowski, J.W. and Handschumacher, R.E. (1985) Cancer Res 45, 5364-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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