R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
uPAR (D4Q5S) Rabbit mAb #12863
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
uPAR (D4Q5S) Rabbit mAb recognizes endogenous levels of total uPAR protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr108 of human uPAR protein.
Background
The human urokinase-type plasminogen activator receptor (uPAR) is a 55-65 kDa, highly glycosylated, GPI-anchored cell surface receptor (the deglycosylated protein is 35 kDa) (1-3). It is a central player in the plasminogen activation pathway. uPAR binds with high affinity to a serine protease urokinase-type plasminogen activator (uPA) and converts plasminogen to its active form plasmin in a spatially restricted manner on the cell surface (4). Plasmin further carries out the activation of uPA, which is inhibited by serpins, such as plasminogen activator inhibitors (5). Therefore, uPAR plays a key role in regulating extracellular proteolysis. In addition, uPAR plays an important role in regulating cell proliferation, adhesion and mobility (6,7). Research studies have shown that overexpression of uPAR is found in various cancer cells and tissues (8,9).
- Nielsen, L.S. et al. (1988) J Biol Chem 263, 2358-63.
- Behrendt, N. et al. (1990) J Biol Chem 265, 6453-60.
- Roldan, A.L. et al. (1990) EMBO J 9, 467-74.
- Ellis, V. et al. (1991) J Biol Chem 266, 12752-8.
- Ellis, V. et al. (1990) J Biol Chem 265, 9904-8.
- Liu, D. et al. (2002) Cancer Cell 1, 445-57.
- Waltz, D.A. et al. (1997) J Clin Invest 100, 58-67.
- Blasi, F. and Sidenius, N. (2010) FEBS Lett 584, 1923-30.
- Mazar, A.P. et al. (2011) Curr Pharm Des 17, 1970-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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