R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UNC93B1 (E6C3E) Rabbit mAb #93568
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UNC93B1 (E6C3E) Rabbit mAb recognizes endogenous levels of total UNC93B1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr40 of human UNC93B1 protein.
Background
Signaling by Toll-like receptors (TLRs) is essential for innate and adaptive immune responses (1-3). The endoplasmic reticulum (ER) protein, UNC93B1, was initially identified by a genetic screen analyzing defects in TLR signaling (1,2). UNC93B1 is a multipass transmembrane protein required for normal TLR function and facilitates trafficking of TLRs from the ER to endolysosomes (4,5). UNC93B1 is essential for signaling of TLR3, TLR7, and TLR9 in both humans and mice, and physically interacts with these TLRs in the ER via adaptor protein complex 2 (AP-2) and syntenin-1 (4-7). Mice carrying mutations in UNC93B1 are susceptible to viral infection and autoimmune disease, and mutations in several regions of UNC93B1 in humans have been implicated in autoimmune diseases such as systemic lupus erythematosus, highlighting the role of UNC93B1 in innate and autoimmunity (7-9).
- Tabeta, K. et al. (2006) Nat Immunol 7, 156-64.
- Kim, Y.M. et al. (2008) Nature 452, 234-8.
- Pelka, K. et al. (2014) J Immunol 193, 3257-61.
- Lee, B.L. et al. (2013) Elife 2, e00291.
- Fukui, R. et al. (2011) Immunity 35, 69-81.
- Majer, O. et al. (2019) Nature 575, 366-370.
- Pelka, K. et al. (2018) Immunity 48, 911-922.e7.
- Majer, O. et al. (2019) Nature 575, 371-374.
- Zhu, H. et al. (2022) J Med Virol 94, 4490-4501.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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