R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UFL1 (F5P6L) Rabbit mAb #96396
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UFL1 (F5P6L) Rabbit mAb recognizes endogenous levels of total UFL1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFL1 protein.
Background
The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4).
- Tatsumi, K. et al. (2010) J Biol Chem 285, 5417-27.
- Gerakis, Y. et al. (2019) Trends Cell Biol 29, 974-986.
- Jiang, Q. et al. (2023) Front Endocrinol (Lausanne) 14, 1123124.
- Jing, Y. et al. (2022) Cancers (Basel) 14, 3501.
- Liu, J. et al. (2017) Nat Commun 8, 14186.
- Walczak, C.P. et al. (2019) Proc Natl Acad Sci USA 116, 1299-1308.
- Liu, J. et al. (2020) Nat Cell Biol 22, 1056-1063.
- Lee, L. et al. (2021) Sci Adv 7, eabc7371.
- Qin, B. et al. (2019) Nat Commun 10, 1242.
- Yoo, H.M. et al. (2014) Mol Cell 56, 261-274.
- Yang, R. et al. (2019) Development 146, dev169235. doi: 10.1242/dev.169235.
- Ishimura, R. et al. (2022) Nat Commun 13, 7857.
- Liang, J.R. et al. (2020) Cell 180, 1160-1177.e20.
- DeJesus, R. et al. (2016) Elife 5, e17290. doi: 10.7554/eLife.17290.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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