R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UFL1 (E3P1Y) Rabbit mAb #39526
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UFL1 (E3P1Y) Rabbit mAb recognizes endogenous levels of total UFL1 protein.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro246 of human UFL1 protein.
Background
The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4).
UFL1 has also been found to promote innate immune responses by regulating the stability of STING independent of UFMylation (15).
UFL1 has also been found to promote innate immune responses by regulating the stability of STING independent of UFMylation (15).
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- Walczak, C.P. et al. (2019) Proc Natl Acad Sci USA 116, 1299-1308.
- Liu, J. et al. (2020) Nat Cell Biol 22, 1056-1063.
- Lee, L. et al. (2021) Sci Adv 7, eabc7371.
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- Yoo, H.M. et al. (2014) Mol Cell 56, 261-274.
- Yang, R. et al. (2019) Development 146, dev169235. doi: 10.1242/dev.169235.
- Ishimura, R. et al. (2022) Nat Commun 13, 7857.
- Liang, J.R. et al. (2020) Cell 180, 1160-1177.e20.
- DeJesus, R. et al. (2016) Elife 5, e17290. doi: 10.7554/eLife.17290.
- Tao, Y. et al. (2023) Cell Death Differ 30, 16-26.
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