UBQLN2 Antibody #14653
Inquiry Info. # 14653
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Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UBQLN2 Antibody recognizes endogenous levels of total UBQLN2 protein. This antibody does not cross-react with other UBQLN proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN2 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Ubiquilin 2 (UBQLN2) is a broadly expressed member of the ubiquilin family of ubiquitin receptor proteins. UBQLN2 is a type 2 ubiquitin-like (UBL) protein that contains an amino-terminal UBL domain, multiple heat shock chaperonin-binding (STI) motifs, several PXX repeats, and a carboxy-terminal ubiquitin-associated (UBA) domain (1-3). Research studies indicate that the UBL domain of UBQLN2 can interact with proteasome subunits (4). The UBA domain of UBQLN2 can interact with ubiquitinated proteins and the autophagosome and allows UBQLN2 to participate in the ubiquitin-proteasome and autophagy pathways (5-8). Mutations in the PXX repeat region of the corresponding UBQLN2 gene are associated with an X-linked form of amyotrophic lateral sclerosis (ALS15) and dementia with reduced penetrance in females (9).
- Wu, A.L. et al. (1999) Mol Cell 4, 619-25.
- Kaye, F.J. et al. (2000) FEBS Lett 467, 348-55.
- Mah, A.L. et al. (2000) J Cell Biol 151, 847-62.
- Kleijnen, M.F. et al. (2000) Mol Cell 6, 409-19.
- Wilkinson, C.R. et al. (2001) Nat Cell Biol 3, 939-43.
- N'Diaye, E.N. et al. (2009) EMBO Rep 10, 173-9.
- N'Diaye, E.N. et al. (2009) Autophagy 5, 573-5.
- Lee, D.Y. et al. (2013) EMBO Rep 14, 373-81.
- Deng, H.X. et al. (2011) Nature 477, 211-5.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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