R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UBQLN1 (D3T7F) Rabbit mAb #14526
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 66 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UBQLN1 (D3T7F) Rabbit mAb recognizes endogenous levels of total UBQLN1 protein. This antibody does not cross-react with other UBQLN proteins.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBQLN1 protein.
Background
Ubiquilin 1 (UBQLN1) is a ubiquitously expressed, type 2 ubiquitin like (UBL) protein that contains an amino-terminal UBL domain and a carboxy-terminal Ub-associated (UBA) domain (1). Research studies demonstrate that UBQLN1 associates with poly-Ub chains through its UBA domain, while the UBL domain participates in interactions with proteasome subunits. Evidence suggests that UBQLN1 acts as a shuttling factor during endoplasmic-reticulum-associated protein degradation (ERAD) as it transports misfolded, ubiquitinated proteins from the ER to the proteasome for subsequent degradation (2-5). Additional research studies demonstrate that the UBL domain of UBQLN1 binds UIM-containing endocytic proteins and participates in the sequestration of protein aggregates during aggresome formation (6,7). UBQLN1 regulates presenilin protein levels and is localized in neurofibrillary tangles of Alzheimer's disease-affected brains (8). Polymorphisms in the corresponding UBQLN1 gene may be associated with a risk of Alzheimer's disease (9-11).
- Hanaoka, E. et al. (2000) J Hum Genet 45, 188-91.
- Ko, H.S. et al. (2004) FEBS Lett 566, 110-4.
- Lim, P.J. et al. (2009) J Cell Biol 187, 201-17.
- Kleijnen, M.F. et al. (2000) Mol Cell 6, 409-19.
- Zhang, D. et al. (2008) J Mol Biol 377, 162-80.
- Heir, R. et al. (2006) EMBO Rep 7, 1252-8.
- Regan-Klapisz, E. et al. (2005) J Cell Sci 118, 4437-50.
- Mah, A.L. et al. (2000) J Cell Biol 151, 847-62.
- Yue, Z. et al. (2014) Int J Neurosci [Epub ahead of print ].
- Viswanathan, J. et al. (2011) Traffic 12, 330-48.
- Bertram, L. et al. (2005) N Engl J Med 352, 884-94.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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