R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb #13439
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 44 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Ubiquityl-PCNA (Lys164) (D5C7P) Rabbit mAb recognizes endogenous levels of PCNA protein only when ubiquitinated at Lys164.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys164 of human PCNA protein.
Background
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.
PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5).
PCNA is mono-ubiquitinated at Lys164 in response to DNA damage at stalled replication forks. This modification is important in the translesion synthesis (TLS) DNA repair pathway (reviewed in 4,5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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