渲染靶标:SSR
Render Timestamp: 2025-02-24T18:42:02.233Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-09-30 01:53:36.431
Product last modified at: 2025-01-01T09:06:18.447Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBE2T (D2L7H) Rabbit mAb #12992

Filter:
  • WB
  • IP
Western Blotting Image 1: UBE2T (D2L7H) Rabbit mAb
Western blot analysis of extracts from various cell lines using UBE2T (D2L7H) Rabbit mAb.

To Purchase # 12992

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 25
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

UBE2T (D2L7H) Rabbit mAb recognizes endogenous levels of total UBE2T protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His150 of human UBE2T protein.

Background

Protein ubiquitination requires the concerted action of the E1, E2, and E3 ubiquitin-conjugating enzymes. Ubiquitin is first activated through ATP-dependent formation of a thiol ester with ubiquitin-activating enzyme E1. The activated ubiquitin is then transferred to a thiol group of ubiquitin-carrier enzyme E2. The final step is the transfer of ubiquitin from E2 to an ε-amino group of the target protein lysine residue, which is mediated by ubiquitin-ligase enzyme E3 (1).

Ubiquitin conjugating-enzyme 2T (UBE2T) is an E2 family member responsible for the ATP-dependent ubiquitin tagging of target proteins for degradation. Research studies indicate that UBE2T plays an important role in the Fanconi anemia pathway and that UBE2T expression is required for normal DNA repair through this pathway. Interaction between UBE2T and FANCL appears to stimulate UBE2T auto monoubiquitination, leading to UBE2T inactivation and negative regulation of the Fanconi anemia pathway (2-4). Additional research details upregulation of UBE2T expression in breast cancer cells and certain lung carcinomas, suggesting a possible involvement in these malignancies (5,6).
For Research Use Only. Not For Use In Diagnostic Procedures.
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