R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
UBAP2L (E5X4E) Rabbit mAb #40199
Filter:
- WB
- IP
- IHC
- IF
- eCLIP
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 150, 160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
UBAP2L (E5X4E) Rabbit mAb recognizes endogenous levels of total UBAP2L protein. This antibody may detect non-specific bands of unknown origin between 80-110 kDa.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human UBAP2L protein.
Background
Ubiquitin-associated protein 2-like (UBAP2L) is a ubiquitous and highly conserved protein containing an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system (UPS) and aggregate formation induced by proteasome inhibitors (1). It can also interact with the Polycomb group protein BMI1 to form a Polycomb subcomplex and regulate hematopoietic stem cell activity (2). UBAP2L is essential for the formation of stress granules (SGs), small cytosolic membraneless organelles that form in eukaryotic cells after stress exposure (3). Some studies suggest that UBAP2L is methylated by the arginine methyltransferase PRMT1, and that this methylation inhibits UBAP2L interaction with SG elements and overall SG assembly (4,5). UBAP2L is also an oncogenic protein found to be amplified and/or overexpressed in a large subset of cancers, including breast cancer, hepatocellular carcinoma, lung adenocarcinoma, glioblastoma, prostate cancer, and colorectal cancer, where it is often associated with tumor growth and poor prognosis (6-12).
- Wilde, I.B. et al. (2011) J Proteome Res 10, 1062-72.
- Bordeleau, M.E. et al. (2014) Blood 124, 2362-9.
- Cirillo, L. et al. (2020) Curr Biol 30, 698-707.e6.
- Maeda, M. et al. (2016) FASEB J 30, 312-23.
- Huang, C. et al. (2020) Cell Death Differ 27, 227-241.
- He, J. et al. (2018) Breast Cancer 25, 224-232.
- Wang, W. et al. (2017) Med Sci Monit 23, 4779-4788.
- Ye, T. et al. (2017) Cell Physiol Biochem 41, 1584-1595.
- Aucagne, R. et al. (2017) FASEB J 31, 5012-5018.
- Zhao, B. et al. (2015) Int J Mol Med 36, 1012-8.
- Li, D. and Huang, Y. (2014) Oncol Rep 32, 1578-84.
- Chai, R. et al. (2016) Tumour Biol 37, 13225-13235.
限制使用
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