R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
U2AF2 (E6M3G) Rabbit mAb #79462
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43, 46, 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
U2AF2 (E6M3G) Rabbit mAb recognizes endogenous levels of total U2AF2 protein. This antibody recognizes U2AF2 protein cleavage products around 43 and 46 kDa.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val275 of human U2AF2 protein.
Background
U2 small nuclear RNA auxiliary factor 2 (U2AF2) is the large (65 kDa) subunit of the U2 auxiliary factor (U2AF) that plays an essential role in the splicing of pre-mRNA to generate functional mRNA transcripts. U2AF2 forms a heterodimer with the small (35 kDa) U2AF1 subunit to create the U2AF that recognizes the 3' splice site and facilitates spliceosome assembly (1-3). Research studies indicate that U2AF1 binds to the 3' splice site consensus AG dinucleotide at the intron-exon boundary while U2AF2 recognizes and binds the polypyrimidine tract upstream of the 3’ splice site. These two steps ensure accurate spliceosome assembly at splice sites (4-6). Mutations in U2AF2, which alter its splicing capabilities, have been described in various cancers (7,8).
- Jurica, M.S. and Moore, M.J. (2003) Mol Cell 12, 5-14.
- Kielkopf, C.L. et al. (2001) Cell 106, 595-605.
- Zamore, P.D. and Green, M.R. (1989) Proc Natl Acad Sci U S A 86, 9243-7.
- Merendino, L. et al. (1999) Nature 402, 838-41.
- Wu, S. et al. (1999) Nature 402, 832-5.
- Zamore, P.D. et al. (1992) Nature 355, 609-14.
- Glasser, E. et al. (2017) Biochemistry 56, 4757-4761.
- Maji, D. et al. (2020) J Biol Chem 295, 17148-17157.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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