Tyrosine Hydroxylase Antibody #2792
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55-60 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Tyrosine Hydroxylase Antibody detects endogenous levels of total tyrosine hydroxylase.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and post-translational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31, and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9).
- Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
- Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
- Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
- Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
- Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
- Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
- Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
- Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
- Saraf, A. et al. (2007) J Biol Chem 282, 573-80.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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