R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TWIST1 (E7E2G) Rabbit mAb #69366
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 26 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TWIST1 (E7E2G) Rabbit mAb recognizes endogenous levels of total TWIST1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro24 of human TWIST1 protein.
Background
TWIST1 is a basic helix-loop-helix (BHLH) transcription factor that functions as a master regulator of embryonic morphogenesis and plays essential roles in mesenchymal differentiation and osteogenic determination (1-3). Mutations affecting the BHLH domain of the TWIST1 gene have been associated with Saethre-Chotzen syndrome (SCS), an autosomal dominant craniosynostosis disorder causing craniofacial and limb abnormalities (4,5). TWIST1 is upregulated in various human tumors and may play a role in epithelial-mesenchymal transition (EMT) and metastasis (6,7). Upregulation of TWIST1 may contribute to resistance to taxol and microtubule-regulating drugs in tumors (8).
- Thisse, B. et al. (1987) Nucleic Acids Res. 15, 3439-53.
- Lee, M.S. et al. (1999) J. Cell Biochem. 75, 566-77.
- Chen, Z.F. and Behringer, R.R. (1995) Genes Dev. 9, 686-99.
- Howard, T.D. et al. (1997) Nat. Genet. 15, 36-41.
- Connerney, J. et al. (2006) Dev. Dyn. 235, 1345-57.
- Watanabe, O. et al. (2004) Anticancer Res. 24, 3851-6.
- Yang, J. et al. (2004) Cell 117, 927-39.
- Wang, X. et al. (2004) Oncogene 23, 474-82.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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