R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TUT4 (E9D9T) Rabbit mAb #15627
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 205 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TUT4 (E9D9T) Rabbit mAb recognizes endogenous levels of total TUT4 protein. This antibody recognizes 82 kDa and 65 kDa bands of unknown origin.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His12 of human TUT4 protein.
Background
Terminal uridylyl transferase 4 and 7 (TUT4/7), also known as ZCCHC11 and ZCCHC6 respectively, are enzymes that posttranscriptionally uridylate mRNAs and miRNAs at the 3’ end (1,2). The TUT enzymes can monouridylate double-stranded RNA with a single nucleotide overhang, which in turn creates a recognition site for Dicer to process miRNAs (3). Many of the miRNAs recognized by TUT4/7 are involved in cell differentiation such as the HOX gene cluster (4). TUT4 and TUT7 have been shown to be recruited by Lin28 in stem cells to uridylate and block expression of let-7 miRNA, a microRNA with tumor suppressive qualities (3,5,6). TUT4 and TUT7 play a role in the mRNA decay pathway, where they polyuridylate targeted mRNAs to promote turnover (7). TUT4 and TUT7 have also been implicated in T cell activation, innate immune response, and antiviral defenses (8-10). Both TUT proteins have also been shown to prevent retrotransposition of LINE-1 elements, indicating a role in maintaining genomic stability (11).
- Rissland, O.S. et al. (2007) Mol Cell Biol 27, 3612-24.
- Wyman, S.K. et al. (2011) Genome Res 21, 1450-61.
- Heo, I. et al. (2012) Cell 151, 521-32.
- Thornton, J.E. et al. (2014) Nucleic Acids Res 42, 11777-91.
- Thornton, J.E. et al. (2012) RNA 18, 1875-85.
- Kim, B. et al. (2015) EMBO J 34, 1801-15.
- Lim, J. et al. (2014) Cell 159, 1365-76.
- Gutiérrez-Vázquez, C. et al. (2017) RNA 23, 882-891.
- Kozlowski, E. et al. (2017) PLoS One 12, e0179797.
- Le Pen, J. et al. (2018) Nat Struct Mol Biol 25, 778-786.
- Warkocki, Z. et al. (2018) Cell 174, 1537-1548.e29.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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