TTF-I Antibody #92145
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TTF-I Antibody recognizes endogenous levels of total TTF-I protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His34 of human TTF-I protein. Antibodies are purified by peptide affinity chromatography.
Background
Transcription termination factor I (TTF-I) is an essential protein that controls the RNA Polymerase I transcription of the rDNA genes. TTF-I binds to the Sal Box, a repetitive sequence element in the spacer region downstream of the 3’ ends of the genes. Binding of TTF-I to this element can pause the elongation complex through the activity of chromatin remodeling complexes, such as NoRC (1-5). TTF-I also has an activating function by binding an upstream terminator element to induce chromatin remodeling (6). TTF-I localization can be controlled by key tumor suppressor ARF, which binds to the TTF-I nucleolar localization sequence, sending it to the nucleoplasm. This process drastically reduces rRNA synthesis (7). Furthermore, TTF-I can be ubiquitinylated by MDM2, which targets it for degradation (8). TTF-I has also been identified to play a role in HIV-1 replication (9).
- Bartsch, I. et al. (1988) Mol Cell Biol 8, 3891-7.
- Evers, R. and Grummt, I. (1995) Proc Natl Acad Sci U S A 92, 5827-31.
- Lang, W.H. et al. (1994) Cell 79, 527-34.
- Mason, S.W. et al. (1997) J Mol Biol 268, 229-34.
- Strohner, R. et al. (2004) Mol Cell Biol 24, 1791-8.
- Längst, G. et al. (1997) EMBO J 16, 760-8.
- Lessard, F. et al. (2010) Mol Cell 38, 539-50.
- Lessard, F. et al. (2012) Nucleic Acids Res 40, 5357-67.
- Park, S.H. et al. (2018) BMB Rep 51, 338-343.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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