R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRMT6 (E7L3M) Rabbit mAb #18791
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 62 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRMT6 (E7L3M) Rabbit mAb recognizes endogenous levels of total TRMT6 protein.
Species Reactivity:
Human, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro333 of human TRMT6 protein.
Background
TRMT6 and TRMT61A, formerly known as GCD10 and GCD14 in yeast, are essential, conserved proteins that form a heterodimer responsible for N1-methylation (m1A) of adenosine 58 in tRNA (1-4). TRMT6 is an RNA-binding protein that recognizes target substrates, while TRMT61A binds AdoMet to catalyze the methylation event (4). TRMT6/61A can additionally produce the m1A mark in small RNAs such as tRNA-derived fragments (tRFs) and also in mRNAs, though in much lower abundance (5,6). The heterodimeric complex is localized to stress granules during heat shock and provides protection for the transcriptome (7). TRMT6 has shown to play a role in progression of malignant gliomas (8). Disruption of the heterodimer has been shown to negatively affect T cell expansion in mice (9).
- Garcia-Barrio, M.T. et al. (1995) Genes Dev 9, 1781-96.
- Anderson, J. et al. (1998) Genes Dev 12, 3650-62.
- Calvo, O. et al. (1999) Mol Cell Biol 19, 4167-81.
- Anderson, J. et al. (2000) Proc Natl Acad Sci U S A 97, 5173-8.
- Safra, M. et al. (2017) Nature 551, 251-255.
- Su, Z. et al. (2022) Nat Commun 13, 2165.
- Alriquet, M. et al. (2021) J Mol Cell Biol 12, 870-880.
- Wang, B. et al. (2021) Front Mol Biosci 8, 692130.
- Liu, Y. et al. (2022) Nat Immunol 23, 1433-1444.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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