R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRIM5α (D6Z8L) Rabbit mAb #14326
Filter:
- WB
- IP
Western blot analysis of extracts from various cell lines using TRIM5α (D6Z8L) Rabbit mAb.
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 56 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRIM5α (D6Z8L) Rabbit mAb recognizes endogenous levels of total TRIM5α protein. This antibody does not react with human TRIM5β and is not predicted to react with other human TRIM5 isoforms based on the location of the antigen.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro395 of human TRIM5α protein.
Background
TRIM5α is a retroviral restriction factor that was originally identified as an HIV restriction factor in Old World monkeys (1). The restriction specificity of TRIM5α varies between species (2). Human TRIM5α only weakly restricts HIV, but efficiently restricts N-tropic murine leukemia virus (N-MLV) (1-3). TRIM5α is composed of a tripartite motif containing RING, B-box 2, and coiled-coil domains, and a B30.2/SPRY domain (4). A 13 amino acid stretch of the B30.2/SPRY domain containing multiple positively charged residues was found to be essential for viral restriction and responsible for variations across species in restriction specificity (4,5). TRIM5α blocks viral infection by interacting with the incoming viral capsid and promoting its premature disassembly (1,6,7). In addition, TRIM5α, together with UBC13-UEV1A, promotes innate immune signaling by catalyzing the synthesis of K63-linked ubiquitin chains that activate TAK1, AP-1, and NF-κB (8).
- Stremlau, M. et al. (2004) Nature 427, 848-53.
- Hatziioannou, T. et al. (2004) Proc Natl Acad Sci U S A 101, 10774-9.
- Yap, M.W. et al. (2004) Proc Natl Acad Sci U S A 101, 10786-91.
- Javanbakht, H. et al. (2005) J Biol Chem 280, 26933-40.
- Sawyer, S.L. et al. (2005) Proc Natl Acad Sci U S A 102, 2832-7.
- Stremlau, M. et al. (2006) Proc Natl Acad Sci U S A 103, 5514-9.
- Chatterji, U. et al. (2006) J Biol Chem 281, 37025-33.
- Pertel, T. et al. (2011) Nature 472, 361-5.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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