R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRIM37 (D7U2L) Rabbit mAb #96167
Filter:
- WB
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRIM37 (D7U2L) Rabbit mAb recognizes endogenous levels of total TRIM37 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His511 of human TRIM37 protein.
Background
Tripartite motif containing protein 37 (TRIM37, also known as MUL1) is a member of the tripartite motif (TRIM) family whose members contain a RING domain, a B-box, and a coiled-coil region (together called RBCC). TRIM37 was initially described as the defining truncation mutation in mulibrey nanism (1). An E3 ubiquitin ligase, TRIM37 is responsible for the monoubiquitination of histone H2A at Lys119, and is amplified in over 40% of breast cancers (2,3). TRIM37 interacts with polycomb group proteins EZH2 and SUZ12, and contributes to histone H3K27 lysine tri-methylation and subsequent silencing of tumor suppressor genes (3). Oncogenic activity of TRIM37 has also been reported in pancreatic, hepatic, and colorectal cancers (4-7).
- Avela, K. et al. (2000) Nat Genet 25, 298-301.
- Kallijärvi, J. et al. (2005) Exp Cell Res 308, 146-55.
- Bhatnagar, S. et al. (2014) Nature 516, 116-20.
- Jiang, J. et al. (2015) Biochem Biophys Res Commun 464, 1120-7.
- Jiang, J. et al. (2016) Tumour Biol 37, 2629-34.
- Zhao, P. et al. (2017) Oncol Res 25, 115-122.
- Hu, C.E. and Gan, J. (2017) Mol Med Rep 15, 1057-1062.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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