R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRIM27 (D5S4O) Rabbit mAb #15099
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRIM27 (D5S4O) Rabbit mAb recognizes endogenous levels of total TRIM27 protein. This antibody also cross-reacts with an unidentified protein of 80 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TRIM27 protein.
Background
Tripartite motif containing protein 27 (TRIM27, RFP) is a member of the tripartite motif (TRIM) family whose members contain a RING domain, a B-box, and a coiled-coil region (together called RBCC). TRIM27 was originally discovered as part of an oncogenic DNA rearrangement resulting in a fusion of the amino terminal RBCC region of TRIM27 with the carboxyl terminal kinase domain of the receptor tyrosine kinase Ret (1). Overexpression of TRIM27 induces JNK and p38 MAPK activation as well as apoptosis (2). TRIM27 has been found to have pleiotropic effects including transcriptional repression (3,4), and E3 ligase activity for ubiquitin (5-7), and SUMO (8). TRIM27 was originally found to interact with Enhancer of Polycomb (EPC) and function as a transcriptional repressor (3). Subsequent studies have identified ubiquitin E3 ligase activity in TRIM27 as well as other members of the TRIM family (reviewed in 9). Potential substrates of TRIM27-mediated ubiquitination include class II PI3K-C2β, NOD2, and WASH. Elevated expression of TRIM27 has been observed in several types of cancer, where in some cases it may be a predictor of poor prognosis (10-13).
- Takahashi, M. et al. (1988) Mol Cell Biol 8, 1853-6.
- Dho, S.H. and Kwon, K.S. (2003) J Biol Chem 278, 31902-8.
- Shimono, Y. et al. (2000) J Biol Chem 275, 39411-9.
- Bloor, A.J. et al. (2005) Oncogene 24, 6729-36.
- Cai, X. et al. (2011) Proc Natl Acad Sci U S A 108, 20072-7.
- Zurek, B. et al. (2012) PLoS One 7, e41255.
- Hao, Y.H. et al. (2013) Cell 152, 1051-64.
- Chu, Y. and Yang, X. (2011) Oncogene 30, 1108-16.
- Meroni, G. and Diez-Roux, G. (2005) Bioessays 27, 1147-57.
- Tezel, G.G. et al. (2009) Pathol Res Pract 205, 403-8.
- Tsukamoto, H. et al. (2009) Cancer Sci 100, 1895-901.
- Iwakoshi, A. et al. (2012) Pathol Int 62, 324-30.
- Zoumpoulidou, G. et al. (2012) J Natl Cancer Inst 104, 941-52.
限制使用
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