R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRBP2 (D7C8K) Rabbit mAb #62043
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 44 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRBP2 (D7C8K) recognizes endogenous levels of total TRBP2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro119 of human TRBP2 protein.
Background
Trans-activation response (TAR) RNA binding protein (TRBP2) was initially discovered as a double stranded RNA binding protein (dsRBP) that bound TAR RNA sequences of the HIV-1 virus (1, 2). TRBP2 can bind to and inhibit the phosphorylation of protein kinase PKR, which leads to increased activation of the HIV-1 long terminal repeat (3,4). Along with PACT, TRBP2 is one of the dsRBPs in the RNA-induced silencing complex (RISC), where it plays a critical role in recruiting Ago2 to the miRNA bound by Dicer. (5-8).
- Gatignol, A. et al. (1991) Science 251, 1597-600.
- Kozak, C.A. et al. (1995) Genomics 25, 66-72.
- Park, H. et al. (1994) Proc Natl Acad Sci U S A 91, 4713-7.
- Daher, A. et al. (2001) J Biol Chem 276, 33899-905.
- Chendrimada, T.P. et al. (2005) Nature 436, 740-4.
- Haase, A.D. et al. (2005) EMBO Rep 6, 961-7.
- Maniataki, E. and Mourelatos, Z. (2005) Genes Dev 19, 2979-90.
- Kok, K.H. et al. (2007) J Biol Chem 282, 17649-57.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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