Transportin-1 Antibody #38700
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Transportin-1 Antibody recognizes endogenous levels of total transportin-1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala354 of human transportin-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Transportin-1 (TNPO1), also known as MIP or karyopherin-β2, is a nuclear import protein that binds to nuclear localization signals (NLSs) on various proteins to help shuttle them from the cytoplasm to the nucleus (1-3). While initially discovered to shuttle hnRNP proteins, transportin-1 has also been shown to import other important regulatory proteins, such as c-Fos and ADAR1 (1,4,5). Transportin-1 plays a role in HIV replication, as it imports and helps uncap the capsid protein (6). Transportin-1 has been implicated in familial ALS, due to its impaired binding affinity to mutations in the FUS-NLS region. Reduction in transportin-1 binding correlated with the time of ALS disease progression in these mutants (7). Transportin-1 can also accumulate in FUS inclusions in wild-type FUS ALS (8). Additionally, transportin-1 plays a role in circadian rhythms by importing PER1 to the nucleus (9).
- Fridell, R.A. et al. (1997) J Cell Sci 110 ( Pt 11), 1325-31.
- Rebane, A. et al. (2004) RNA 10, 590-9.
- Pollard, V.W. et al. (1996) Cell 86, 985-94.
- Arnold, M. et al. (2006) J Biol Chem 281, 5492-9.
- Fritz, J. et al. (2009) Mol Cell Biol 29, 1487-97.
- Fernandez, J. et al. (2019) Nat Microbiol 4, 1840-1850.
- Niu, C. et al. (2012) PLoS One 7, e47056.
- Takeuchi, R. et al. (2013) Neuropathol Appl Neurobiol 39, 580-4.
- Korge, S. et al. (2018) PLoS Genet 14, e1007189.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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