R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Transgelin/TAGLN (E6Y8S) Rabbit mAb #52011
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Transgelin/TAGLN (E6Y8S) Rabbit mAb recognizes endogenous levels of total Transgelin/TAGLN protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly51 of human Transgelin/TAGLN protein.
Background
Transgelin (TAGLN, SM22α) is an actin-binding protein of the calponin family. It contains an amino-terminal calponin homology domain and a carboxy-terminal calponin-like module domain required for actin binding (1,2). TAGLN is a TGF-β inducible gene that is abundantly expressed in visceral and vascular smooth muscle cells, and is often used as an early marker of smooth muscle differentiation (3-5). TAGLN expression has also been shown in brain pericytes, providing use in studying the blood brain barrier (6). Altered levels of TAGLN are seen in multiple cancer types, including colorectal, breast, and prostate cancer (4).
- Assinder, S.J. et al. (2009) Int J Biochem Cell Biol 41, 482-6.
- Fu, Y. et al. (2000) J Appl Physiol (1985) 89, 1985-90.
- Hirschi, K.K. et al. (1998) J Cell Biol 141, 805-14.
- Dvorakova, M. et al. (2014) Expert Rev Proteomics 11, 149-65.
- Huang, L. et al. (2018) J Cell Mol Med 22, 6249-6261.
- Blanchard, J.W. et al. (2020) Nat Med 26, 952-963.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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