TRA-1-81 (TRA-1-81) Mouse mAb #4745
Filter:
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200-400 |
| Source/Isotype | Mouse IgM |
Application Key:
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:1600 |
| Flow Cytometry (Live) | 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TRA-1-81 detects endogenous levels of TRA-1-81 antigen.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human embryonal carcinoma 2102Ep cl.2A6 cells.
Background
TRA-1-60 and TRA-1-81 antibodies detect antigens present on the surface of human stem, teratocarcinoma, and embryonic germ cells (1). TRA-1-60(S) reacts with a neuraminidase sensitive epitope of a proteoglycan (2,3), while TRA-1-81 reacts with a neuraminidase insensitive epitope on the same antigen. Recently this antigen has been proposed to be a form of the protein podocalyxin (4). TRA-1-60 is also detected in the serum of patients with germ cell tumors (5,6).
- Andrews, P.W. et al. (1987) Int J Androl 10, 95-104.
- Andrews, P.W. et al. (1991) Recent Results Cancer Res 123, 63-83.
- Badcock, G. et al. (1999) Cancer Res 59, 4715-9.
- Schopperle, W.M. and DeWolf, W.C. (2007) Stem Cells 25, 723-30.
- Thomson, J.A. et al. (1998) Science 282, 1145-7.
- Marrink, J. et al. (1991) Int J Cancer 49, 368-72.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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