R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TR4/NR2C2 (E1T8N) Rabbit mAb #31646
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 67 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TR4/NR2C2 (E1T8N) Rabbit mAb recognizes endogenous levels of total TR4/NR2C2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile10 of human TR4/NR2C2 protein.
Background
Testicular receptor 4 (TR4), also called TAK1 or NR2C2, is an orphan receptor in a distinct subclass of the steroid hormone nuclear receptor superfamily along with TR2 (1,2). TR4 forms heterodimers with TR2 and binds to DNA elements containing direct repeats (DRs) (3). TR4 plays a role in various processes, including neurogenesis, spermatogenesis, RNA metabolism, and protein translation (4-6). TR4 can interact with other hormone receptors such as AR and ER to influence protein-protein binding and target gene inactivation (7,8). High expression of TR4 correlates with prostate cancer metastasis and invasion through downstream targets such as CCL2 and Ezh2 (9-10). Expression of TR4 in other cancers such as NSCLC and testicular germ cell tumors has also been associated with poor prognosis (11-12).
- Chang, C. et al. (1994) Proc Natl Acad Sci U S A 91, 6040-4.
- Law, S.W. et al. (1994) Gene Expr 4, 77-84.
- Lee, C.H. et al. (1998) J Biol Chem 273, 25209-15.
- Young, W.J. et al. (1998) J Biol Chem 273, 20877-85.
- Mu, X. et al. (2004) Mol Cell Biol 24, 5887-99.
- O'Geen, H. et al. (2010) BMC Genomics 11, 689.
- Shyr, C.R. et al. (2002) J Biol Chem 277, 14622-8.
- Lee, Y.F. et al. (1999) Proc Natl Acad Sci U S A 96, 14724-9.
- Ding, X. et al. (2015) Int J Cancer 136, 955-64.
- Zhu, J. et al. (2015) Mol Cancer Ther 14, 1445-53.
- Zhang, L. et al. (2015) Lung Cancer 89, 320-8.
- Chen, Y. et al. (2018) Cancer Sci 109, 384-394.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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