R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TPPP/p25 (E7F8S) Rabbit mAb #51412
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TPPP/p25 (E7F8S) Rabbit mAb recognizes endogenous levels of total TPPP/p25 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TPPP/p25 protein.
Background
Tubulin polymerization-promoting protein (TPPP, also known as p25) is a microtubule nucleation factor in oligodendrocytes. As a largely unstructured protein, TPPP/p25 induces microtubule polymerization in vitro (1,2). TPPP/p25 is enriched in oligodendrocytes in the brain and may play a unique role in the structure and function of these unique cells (3,4). Oligodendrocytes extend elaborate microtubule arbors that contact neuronal axon segments to generate a myelin sheath that is critical for proper propagation of axonal action potentials. TPPP/p25 may not only play a critical role in establishing the microtubule network for oligodendrocytes, but may also contribute to the generation of Golgi outposts, which are Golgi found outside of the cell body. Golgi outposts may play a specialized role in building the local cytoarchitecture of oligodendrocytes and other brain cells (5,6). Misregulation of TPPP/p25 is linked to several neurodegenerative diseases, including Parkinson’s disease, and may contribute to disease etiology and/or represent a potential therapeutic target (7).
- Hlavanda, E. et al. (2002) Biochemistry 41, 8657-64.
- Hlavanda, E. et al. (2007) J Biol Chem 282, 29531-9.
- Takahashi, M. et al. (1991) FEBS Lett 289, 37-43.
- Takahashi, M. et al. (1993) J Neurochem 60, 228-35.
- Fu, M.M. et al. (2019) Cell 179, 132-146.e14.
- Valenzuela, A. et al. (2020) Trends Cell Biol 30, 792-804.
- Oláh, J. et al. (2017) Expert Rev Proteomics 14, 301-309.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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