R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TNIK (F7V3J) Rabbit mAb #90389
Filter:
- WB
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TNIK (hTNIK-Myc/DDK; +), using TNIK (F7V3J) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 180 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:250 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TNIK (F7V3J) Rabbit mAb recognizes endogenous levels of total TNIK protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg567 of human TNIK protein.
Background
TRAF2 and NCK-interacting protein kinase (TNIK) is a member of the germinal center kinase (GCK) family (1). TNIK phosphorylates TCF4 and is an essential activator for Wnt signaling (2). Animal knockout model and kinase inhibition studies have reported that TNIK can stimulate both cancer cell growth and epithelial-mesenchymal transition (EMT) (3-5). TNIK has also been shown to promote F-actin disruption through its interactions with Rap2 (6). In neuronal cells, TNIK is enriched in the postsynaptic density (PSD), where it is reported to modulate neuronal receptor surface expression and dendrite complexity and signaling (7-9).
- Fu, C.A. et al. (1999) J Biol Chem 274, 30729-37.
- Mahmoudi, T. et al. (2009) EMBO J 28, 3329-40.
- Masuda, M. et al. (2016) Nat Commun 7, 12586.
- Kim, J. et al. (2014) PLoS One 9, e110180.
- Shitashige, M. et al. (2010) Cancer Res 70, 5024-33.
- Taira, K. et al. (2004) J Biol Chem 279, 49488-96.
- Hussain, N.K. et al. (2010) J Neurosci 30, 14786-94.
- Coba, M.P. et al. (2012) J Neurosci 32, 13987-99.
- Larhammar, M. et al. (2017) J Neurosci 37, 11074-11084.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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