TNIK Antibody #32712
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 180 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
TNIK Antibody recognizes endogenous levels of total TNIK protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg567 of human TNIK protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
TRAF2 and NCK-interacting protein kinase (TNIK) is a member of the germinal center kinase (GCK) family (1). TNIK phosphorylates TCF4 and is an essential activator for Wnt signaling (2). Animal knockout model and kinase inhibition studies have reported that TNIK can stimulate both cancer cell growth and epithelial-mesenchymal transition (EMT) (3-5). TNIK has also been shown to promote F-actin disruption through its interactions with Rap2 (6). In neuronal cells, TNIK is enriched in the postsynaptic density (PSD), where it is reported to modulate neuronal receptor surface expression and dendrite complexity and signaling (7-9).
- Fu, C.A. et al. (1999) J Biol Chem 274, 30729-37.
- Mahmoudi, T. et al. (2009) EMBO J 28, 3329-40.
- Masuda, M. et al. (2016) Nat Commun 7, 12586.
- Kim, J. et al. (2014) PLoS One 9, e110180.
- Shitashige, M. et al. (2010) Cancer Res 70, 5024-33.
- Taira, K. et al. (2004) J Biol Chem 279, 49488-96.
- Hussain, N.K. et al. (2010) J Neurosci 30, 14786-94.
- Coba, M.P. et al. (2012) J Neurosci 32, 13987-99.
- Larhammar, M. et al. (2017) J Neurosci 37, 11074-11084.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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